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Maximum PC magazine did an article on a DIY NAS with it's own dedicated NAS OS.
I found the article very informative and downloaded the ISO. I think it's the best option when you look at all the streaming support for different clients and even lets you share your iTunes library across the LAN to any PC.
Check it out.
Using FreeNAS. You install it on a computer, do the initial configuration, and then log into a web interface to do just about anything you want. It's basically a web GUI to manage a FreeBSD server.